Why topoisomerase (I and II) assays are performed using drug concentration of 50 or 100 micro-molars?

I have consulted multiple publication where Topoisomerase assays are performed using drug concentration of 50 and 100 micromolar? why such high concentration used for this assay? Is there any...

05 June 2019 5,962 1 View

What is most reliable method for testing the antileishmanial activity induced by small molecule inhibitors in vitro using promastigotes culture?

The literature reveals array of assay including assay using tetrazolium dyes, using mix of sybr green in Triton, counting using cytometer, etc. I would appreciate if the expert working in said...

04 May 2019 3,148 3 View

HRMS data showing mass less than 16 amu from the exact mass?

Hi, I am analysing my HRMS spectral data, and I observed my mass to be 457.1980, which is having 16 amu difference from the calculated mass 473.2216 (Chemical Formula: C30H27N5O). I have duly...

05 June 2018 6,433 21 View

Is compensation done in flow cytometry can be applied to single sample or is it mandatory to apply the same compensation to all samples under study?

I am using two dyes in flow cytometer and they spill over in some of the samples, so should i apply compensation to only those individual samples having spill over or it is mandatory to apply the...

03 April 2018 3,997 2 View

How to synthesize N-formyl containing molecules using deuterated formic acid (DCOOH)?

I have been synthesizing N-formylated compounds previously using formic acid, but when the formic acid was replaced with deuterated formic acid reaction is not progressing under similar...

02 March 2018 1,507 5 View

What will the effect of Xanthine Oxidase Inhibition on HIF-alpha gene expression?

In my experiment i am getting increased expression of HIF-1alpha gene after the qPCR experiments. the results are consistent with allopurinol and other synthetic molecule. Kindly help me with...

11 December 2017 7,101 2 View

Is it necessary that VEGF gene expression decreases every time with anticancer drug treatment?

VEGF has been related to angiogenesis in cancer and it expression is reported to decrease after treatment with a potent anticancer agents. In my experiment (gene expression) i am failing to get...

10 November 2017 1,595 7 View

What are the various in vitro and in silico studies need to be done for the assessment of toxicity profile of a synthetic molecule?

Any research papers, protocols based on mentioned theme will be helpful.

10 November 2016 4,287 0 View

Which is the best fixing solvent or reagent used to increase permeability of nuclear membrane for Propidium iodide?

31 December 2015 8,009 3 View

Is there any correlation between EGFR and HDAC on receptor basis?

A drug currently developed to target EGFR-HDAC, CUDC-101 is in phase -1 trial and claims to show dual inhibitory activity on both EGFR and HDAC

07 August 2014 1,016 2 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

How to write a scientific report?

We are analysing scientific reports at University in great detail, so I wanted to find a scientific report about how to write a scientific report. Including detail such as what information to put...

02 March 2021 7,602 4 View

How to get started building and analyzing a UTAUT model?

Hello all, I need help getting started building and analyzing a UTATU model. I have a survey ready with some questions but I had some confusions on UATAUT and on how to analyze the data. I will...

02 March 2021 9,271 1 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

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01 March 2021 3,905 5 View

How to Balance Bench work with Reading and Writing?

I am a second-year grad student. I am finding it difficult finding time to read literature and write (things like qualifying exam, thesis, grants). I find myself so busy with bench work that I...

01 March 2021 3,325 4 View

Could anyone tell me what is the range of submillimeter wave?

There is confusion regarding the submillimeter-wave range. Many papers specify its range from 300GHz but some also consider its range from 100GHz. If I design an antenna on 122ghz what would I...

01 March 2021 516 7 View

Which software is better to write systematic review paper?

We have seen some authors use some software to write review paper. As obvious, the study of all published papers concerning our studying field is not possible and these software can help us to...

27 February 2021 6,296 1 View