Is there any cell line that constitutively expresses dystrophin?

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What does a decreasing 𝐶𝑒 vs. 𝑄e plot indicate in an adsorption isotherm?

I am conducting adsorption experiments in a liquid medium, and one of my samples showed a decrease in Qe values with an increase in Ce. Has anyone encountered this behavior before? What does it...

26 June 2024 7,304 2 View

Expert in advanced statistics interested in contributing to an academic article on the adoption of digital innovations in agriculture?

We are looking for a highly qualified researcher with expertise in advanced statistical analysis to contribute to a scientific article to be submitted to a prestigious journal by the end of the...

13 June 2024 569 4 View

Word error in a title / full text (1 page) shared: may I correct it ?

A recently published visual abstract should have the title "Word First Myelography..." instead of the error "Mielography". (the 2 other text references in the article are correct) I am changing it...

20 May 2024 8,134 1 View

Fluorescence spectroscopy: signal from water?

In order to proceed with the quantification of an Azide Alkyne Cycloaddition reaction efficiency by using a fluorogenic probe with Cy5-DBCO, I first need to construct a calibration curve. The...

16 May 2024 6,266 5 View

Is there a commercially available cell line from human cancer-associated fibroblasts (CAFs) isolated from malignant pleural mesothelioma or lung ca ?

Dear community, I am currently working on a proposal focusing on cancer-associated fibroblasts (CAFs) from malignant pleural mesothelioma (MPM). In my search among commercial cell line providers,...

05 May 2024 5,572 4 View

For DOM (dissolved organic matter) and in addition to the Follin method strategy, is there another way to determine phenolic content in the sample?

This is working with DOM (dissolved organic matter) and in addition to the acid-base titration strategy, Follin's method, there is another strategy to determine the amount of phenolic?

23 April 2024 2,197 1 View

Could you use a Tesla Turbine in a car?

In 1913, Nikola Tesla patented a new kind of turbine that required no blades and converted kinetic fluid energy into rotational energy. It uses nozzles that apply fluid to the edges of smooth...

17 April 2024 6,702 0 View

Where i can find calibration area used from my model ?

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14 April 2024 2,460 3 View

How can I reduce the variability of CFU counts between replicates?

I'm challenging bacteria with different compounds, then plating the suspension on LB agar to assess CFU and understand the bactericidal properties of my compounds. However, I'm encountering...

10 April 2024 7,459 7 View

Perceptions and practices in education?

Hello. Can anyone please ell me what theoretical background I can use to measure perceptions and practices of teachers in interculturality?

27 March 2024 485 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

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07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

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How to isolate lymphocytes from mouse spleen?

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How to Add Missing Water Molecules in Protein-Membrane Simulations?

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04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

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To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

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03 August 2024 3,851 1 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

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Sandip Chakraborty

Sol cell line constitutively express the BMD mini-dystrophin. You can refer to the following links to get certain valuable informations:-

1. http://www.ncbi.nlm.nih.gov/pubmed/15212940

2. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2151485/#bib28

Sandrine Herbelet

Dear Carlos,

Just for info: when growing skeletal cell lines, dystrophin is only present at the sarcolemma after three weeks. I always grow my cell lines longer than three weeks.

What does a decreasing 𝐶𝑒 vs. 𝑄e ​plot indicate in an adsorption isotherm?