What is the best way to do homogenization for small intestine for doing western blotting?

01 February 2015 2,736 9 View

Why is the C-peptide used generally instead of insulin in detection and qualification of functional beta cell situation?

10 November 2014 7,084 21 View

Is there any method to determine miRNA-miRNA interactions in the cytoplasm?

10 November 2014 586 4 View

Why has the 16S rRNA undergone slightly alteration? What is the protective mechanism for it ?

09 October 2014 7,887 4 View

How can I detect the pancreatic adenocarcinoma in cell culture ?

08 September 2014 854 6 View

Why in the beginning and ending portions, approximately 200 base sequences of my gene aren't clear as much as the middle parts of my gene?

07 August 2014 9,160 5 View

Does anyone know which ingredients will be inactivated or broken down and so forth at the end of the PCR Thermal Cycler Process ?

First when I did PCR with a new primers I saw many bands on agarose. I adjusted annealing temperature for this PCR process, 50 degree. Subsequently, I prepared new PCR mixture from new cDNAs that...

07 August 2014 8,220 7 View

Can I use the wash buffers which I use in RNA isolation process to purify the my PCR samples in which PCR tubes?

06 July 2014 8,948 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View