Ediz Coşkun

9 Questions 23 Answers 0 Followers

Questions related from Ediz Coşkun

What is the best way to do homogenization for small intestine for doing western blotting?

I have a problem about small intestine in western blotting. My problem is probably homogenization process. In the previous experience that our study group did, we couldn't find the completely...

02 February 2015 2,785 9 View

Is there any cell line center or any person that I can have BAT precursor (known marker myf5+) and brown adipocyte cell line?

I researched on the internet question above, I couldn't find any directing site. In general, scientists had isolated their own BAT precursor ( Myf5+) and Brown adipocyte cells in their...

12 December 2014 1,637 6 View

Is there any method to determine miRNA-miRNA interactions in the cytoplasm?

I know one miRNA regarding my study. On the other hand I think this miRNA is blocked by the another miRNA which I don't know when the conditions changed. İs there any method to identify the new...

11 November 2014 633 4 View

Why is the C-peptide used generally instead of insulin in detection and qualification of functional beta cell situation?

I know that C-peptide is released with insulin hormone from beta cells. But there must be a facility to the use of C-peptide instead of insulin, because most of the investigations that I examined...

11 November 2014 7,124 21 View

Why has the 16S rRNA undergone slightly alteration? What is the protective mechanism for it ?

As you know, 16S rRNA is used to in phylogenetic classification owing to slow rates evolution of this gene, being in all prokaryotes etc. But which mechanism maintains this situation? Or is...

10 October 2014 7,935 4 View

How can I detect the pancreatic adenocarcinoma in cell culture ?

What are the main markers ?

09 September 2014 896 6 View

Does anyone know which ingredients will be inactivated or broken down and so forth at the end of the PCR Thermal Cycler Process ?

First when I did PCR with a new primers I saw many bands on agarose. I adjusted annealing temperature for this PCR process, 50 degree. Subsequently, I prepared new PCR mixture from new cDNAs that...

08 August 2014 8,265 7 View

Why in the beginning and ending portions, approximately 200 base sequences of my gene aren't clear as much as the middle parts of my gene?

In total, I have a gene which has 896 bases. After DNA sequencing process (as I mentioned in the title), approximately both are on the beginning portion. Almost 100 bases at the beginning portion,...

08 August 2014 9,210 5 View

Can I use the wash buffers which I use in RNA isolation process to purify the my PCR samples in which PCR tubes?

Because I have bright bands in the onset of well of agarose gel. I have a doubt about contamination. I used a control cDNA to verify PCR process. I attained the right Dna fragments as I expected....

07 July 2014 8,995 4 View