We have been using Ketamine + Xylazine as a standard anaesthetic for mice in our lab. However, due to some policy problems, ketamine is no longer produced here in China. I wonder if there is any other anaesthetic that have similar anaesthetic effect on mice? Please help me. Thanks a million!
I have seen my seniors to anesthetize mice by chloroform or ether (di-ethyl ether) or high concentration of CO2. The last one is most perfect to anesthetize.
Try intraperitoneal pentobarbital. 50-60 IU/kg. Other options include isoflurane but the anaesthetic setup is expensive.
thiopental worked also i.p but has limited analgesic effects. Combine with diazepam should be usefull to increase analgesia (but need to applicate in lower dose). Phenobabital and diazepam may also a good solution. Overall anesthesia depend on kind of approach
Isoflurane is good as it can be used for sedating for surgeries and terminal experiments.
Hi, chloroform or ether is no longer used (depending on the countries) because they are too aversive for the animals.
It really depends what kind of procedures do you want to do to your mice. Isoflurane is good, but when the procedure induces pain, you should also give an analgesic as butorphanol or carprofen or fentanyl, as stated before. This procedure would be good for surgeries (don't forget post-operative care) since it provides a controlled level of anaesthesia in a long duration. The limitation is that you have to have a vaporizer, which could be expensive.
For short-procedures when you give a bolus, I agree that you can use barbiturates as pentobarbital, with analgesia. As already stated you can use with barbiturates diazepam or medetomidine.
Propofol is a hypnotic, so you need analgesia and is quite good for surgeries when you can do an IV continuous infusion, however in mice it could be hard. You can also use 75/1/0.2 mg/kg of propofol, medetomidine and fentanyl, respectively, ip route, for short procedures and with a good analgesia (see paper attached).
Hope you can find a good solution for you and your animals.
medetomiidine was extensively used in veterinary anesthesia and dexmedetomidine is regularly used in humans.So it can be tried.
Ketamine/Xylazine/Acepromazine cocktail is safe and effective for short duration anesthesia whereas isoflurane can be used for longer rodent surgery but setup is quite expensive
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