Is there an enzymatic assay for succinate dehydrogenase subunit A (SDHA)?

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Muhammad Waheed Iqbal

Yiming Qin Dear, could you please explain your reaction mixture?

Yiming Qin

I want to measure the SDHA activity (convert succinate to fumarate). I plan to express my mutant SDHA in e.coli, but I don't think there is an assay to measure just subunit A activity.

Adam B Shapiro

Here is a kit for measuring the succinate to fumarate reaction using an artificial acceptor.

https://www.biovision.com/documentation/datasheets/K660.pdf

Here is an article on a coupled enzyme assay:

Article A spectrophotometric coupled enzyme assay to measure the act...

There is also an ELISA kit in case you want to measure the amount of the protein instead of its activity:

https://www.mybiosource.com/mouse-elisa-kits/succinate-dehydrogenase-complex-subunit-a-sdha/2000189

Adam B Shapiro thank you very much Sir, for sharing such a valuable information.

Imran Mahmood Khan

Dear

Have a look on the following website. May it help

https://www.abcam.com/succinate-dehydrogenase-activity-assay-kit-colorimetric-ab228560.html\

https://www.sigmaaldrich.com/china-mainland/zh/life-science/cell-biology/cell-biology-products.html?TablePage=112668420

.... Sdert

There are kits based on this enzyme