Yiming Qin

4 Questions 4 Answers 0 Followers

Questions related from Yiming Qin

OROBOROS data analysis, marks on flux?

I recently did an OROBOROS run, with the result attached. My question: where should I mark the flux for succinate-dependent respiration capacity (chamber A 36-50 min, chamber B 36-60min)? should I...

26 January 2022 6,509 0 View

Can I use saponin to permeabilize cell to measure mitochondrial respiration?

Saponin and digitonin both can permeabilize cells. However, based on many respiration assay protocols I read, saponin is used for tissue permeabilization and digitonin is used for cell...

12 January 2021 2,325 3 View

Is there an enzymatic assay for succinate dehydrogenase subunit A (SDHA)?

Is there an enzymatic assay for SDHA specifically, not SDH activity?

30 September 2019 8,606 6 View

What protease inhibitors should I use if I want to IP a serine protease and test its activity?

I would like to IP LONP1 protease from MCF-7 cell. LONP1 is a mitochondrial serine protease. My question is what protease inhibitor should I use to IP but still maintain my LONP1 activity? I know...

07 March 2018 6,724 5 View