I'm thinking about using an ELISA kit to measure the levels of inflammatory cytokines (e.g. TNF-alpha, IL-10, etc). We have small biopsy material from humans and wants to homeogenize with a RIPA buffer (that we use for our Western Blots).
Do any of you have experience with a kit that does this?
I found one but unfortunately it only work with serum/plasma, not homeogenates because of the detergents in RIPA buffer.
Best regards
L
Hi, you may use the milliplex (from millipore) or bioplex (from Biorad), which work on plasma, serum and tissue or cell lysates. Those kits require a specific machine since they are fluorescent bead based, but you can assay many analytes on one single sample, in the same well. I would check what buffer for homogenization is required for the kit you will end up using prior to processing the samples though. The SDS in RIPA buffer may not be recommanded.
Hope this helps
R an D systems also has an ELISA for this. You can check their protocol. I also don't think SDS is recommended. Just run with standard plate reader.
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