Lars Koch Hansen

6 Questions 23 Answers 0 Followers

Questions related from Lars Koch Hansen

Adjust Cq values for PCR efficiency?

Right now I've tested all my primers to have a PCR efficiency of 94-104% which is acceptable. I've been thinking about adjusting for the efficiency as my reference gene's efficiency is 104%, which...

07 July 2013 4,857 6 View

Contract company to do a small animal study?

Do any of you know whether you can get a research animal facility to perform a small study on rats (edit: not mice)? I really would like to do an ulcerative colitis rat model that are divided into...

06 June 2013 4,398 5 View

Is there a CD marker that tells you that a T-cell is activated?

We want to test whether a patients T-cells are suppressed on different kinds of medications? How can you measure T cell activation/activity? My first thought was whole-cell patchclamping. But what...

10 October 2012 581 15 View

A reviewer rejects your manuscript and then uses your idea and then publishes it afterwards elsewhere?

Let's say that you submit your manuscript to a journal. It goes trough peerreview but then gets rejected. Afterwards you revise the paper and resubmit to another journal and it gets...

08 August 2012 8,498 26 View

Is there an ELISA kit for inflammatory cytokines that work on tissue homeogenates (RIPA buffer)?

I'm thinking about using an ELISA kit to measure the levels of inflammatory cytokines (e.g. TNF-alpha, IL-10, etc). We have small biopsy material from humans and wants to homeogenize with a RIPA...

06 June 2012 7,487 2 View

Is it possible to do Flowcytometry on frozen PBMCs from Ficoll Paque gradient - CD4+/CC7- and CD8+/CCR7- ?

Dear all I'm planning a small FC study. I want to use peripheral blood and use a density gradient to seperate into MCs (Ficoll Paque Plus). My plan was to freeze down these PBMCs at -80...

06 June 2012 9,061 13 View