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Questions related from Lars Koch Hansen
Right now I've tested all my primers to have a PCR efficiency of 94-104% which is acceptable. I've been thinking about adjusting for the efficiency as my reference gene's efficiency is 104%, which...
07 July 2013 4,854 6 View
Do any of you know whether you can get a research animal facility to perform a small study on rats (edit: not mice)? I really would like to do an ulcerative colitis rat model that are divided into...
06 June 2013 4,396 5 View
We want to test whether a patients T-cells are suppressed on different kinds of medications? How can you measure T cell activation/activity? My first thought was whole-cell patchclamping. But what...
10 October 2012 579 15 View
Let's say that you submit your manuscript to a journal. It goes trough peerreview but then gets rejected. Afterwards you revise the paper and resubmit to another journal and it gets...
08 August 2012 8,497 26 View
I'm thinking about using an ELISA kit to measure the levels of inflammatory cytokines (e.g. TNF-alpha, IL-10, etc). We have small biopsy material from humans and wants to homeogenize with a RIPA...
06 June 2012 7,484 2 View
Dear all I'm planning a small FC study. I want to use peripheral blood and use a density gradient to seperate into MCs (Ficoll Paque Plus). My plan was to freeze down these PBMCs at -80...
06 June 2012 9,059 13 View