VMD error file:///usr/local/lib/vmd/vmd_LINUX: not found ?

I tried to download VMD version 1.9 as it wouldn't require things for my computer to process. I did the necessary steps to install it and it finally said "VMD installation complete, Enjoy!"...

06 September 2020 6,224 2 View

What tools do you use for development and training on a remote machine?

Hi, I currently use vscode with the remote add-on for this and a ssh session with tmux. With this setup I can edit files, debug and run scripts remotly. What I use: Python, C++, ROS, Gazebo,...

14 May 2020 3,760 3 View

A reliable protocol for cholesterol staining in fixed tissue?

I am looking for a fluorescent probe or stain that would only bind surface cholesterol and that it is suitable for sections of fixed tissue. I think filipin can be used cells, but I am not sure...

26 December 2019 7,902 4 View

How to report a Confirmatory Factor Analysis for an instrument with 188 items?

I'm currently conducting a CFA in order to validate a psychometric test composed by 188 likert scale items. The theoretic framework of the test states that there are 18 factors that can be...

02 April 2019 9,261 5 View

Where is it more feasible to place a construct for gene expression of recombinant enzymes in P. Infestans (fungus)?

I am in an academic research project, where we want to introduce a gene that codes for obtaining an enzyme that degrades phenols in the organism P. Infestans, so we want to introduce a construct...

29 November 2018 8,460 3 View

Does Promega's Passive Lysis 5X Buffer contains anti-protease and anti-phosphatase components?

Hi everyone, Do you know if the Passive Lysis 5X Buffer that Promega produce has these components? I always use it to lysate transfected cells and measure lucierase activity and now I'm wondering...

03 September 2018 1,322 1 View

QRTPCR amplification of target with low GC content?

Hey everyone. I have a question. I'm doing the in vitro silencing of a small RNA of 79 bp (a snoRNA specifically), and I need to design a protocol for the detection of the known-down of this RNA....

26 December 2016 6,061 2 View

Would like to know colleagues familiar with the programme Geant4?

One of its uses: radiation interaction with cells. C++ required.

06 March 2014 9,288 12 View

Which molecular simulation program could I use for studying Amyloid beta peptide folding pathways?

I am currently using protein structure prediction servers to study A-beta folding pathway. I will like to learn to use molecular simulation programs that can give me a better insight of these...

14 December 2013 8,662 7 View

What is a better way to make a good IV curve in a DSSC system?

10 November 2013 5,088 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View