we have done a PCR for adhesion molecules comparing lung tissue from healthy mice to inflamed mice. surprisingly, the RNA expression in the inflamed mice is much lower than the healthy ones. I don't know how to interpret this, we are ruling out technical problems. Has anyone seen this before?
Katie A S Burnette
Did you normalize the data by comparing to a reference/housekeeping gene?
Not all gene expression is regulated primarily at the transcript level. Can you measure protein abundance?
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