Velmarini Vasquez

3 Questions 2 Answers 0 Followers

Questions related from Velmarini Vasquez

Is there an alternative for proximity ligation assay antibody diluent or homemade antibody diluent recipe suitable for this assay ?

I will like to know if there is a company/homemade alternative to the duo link PLA kit antibody diluent, since this reagent finishes faster than the rest of the reagent provided in the kit.

09 December 2016 5,553 1 View

What should be the composition of cell lysis buffer for extracting alpha synuclein protein from SHSY5Y cells ?

I have been using RIPA lysis buffer (50mM Tris, 150mM NaCl, 1mM EDTA, 1%Triton-X, 1tab protease inhibitor). I am having problems detecting endogenous alpha synuclein so I want to make sure I am...

04 November 2014 1,223 4 View

Which molecular simulation program could I use for studying Amyloid beta peptide folding pathways?

I am currently using protein structure prediction servers to study A-beta folding pathway. I will like to learn to use molecular simulation programs that can give me a better insight of these...

15 December 2013 8,687 7 View