Velmarini Vasquez

5 Questions 4 Answers 0 Followers

Questions related from Velmarini Vasquez

Is there an alternative for proximity ligation assay antibody diluent or homemade antibody diluent recipe suitable for this assay ?

I will like to know if there is a company/homemade alternative to the duo link PLA kit antibody diluent, since this reagent finishes faster than the rest of the reagent provided in the kit.

09 December 2016 5,613 1 View

What should be the composition of cell lysis buffer for extracting alpha synuclein protein from SHSY5Y cells ?

I have been using RIPA lysis buffer (50mM Tris, 150mM NaCl, 1mM EDTA, 1%Triton-X, 1tab protease inhibitor). I am having problems detecting endogenous alpha synuclein so I want to make sure I am...

04 November 2014 1,257 4 View

Which protein stability analysis tool would you recommend to determine the stability values of predicted protein tertiary structures?

I am trying to see the changes of the stability of predicted protein structures after certain amino acids substitution.

18 January 2014 7,584 0 View

Which molecular simulation program could I use for studying Amyloid beta peptide folding pathways?

I am currently using protein structure prediction servers to study A-beta folding pathway. I will like to learn to use molecular simulation programs that can give me a better insight of these...

15 December 2013 8,739 7 View

Which type of protein modeling is better suited for a 3D structure to represent Alzheimer's Amyloid Beta 1-42 fragment, homology or de novo?

I am trying to understand the protein folding pathway of Amyloid Beta 1-42 using secondary structure prediction servers like Phyre 2 and I-Tasser for homology modeling, and Quark and CABS-fold...

13 December 2013 5,357 11 View