So I am trying to live-image the synapses of NK cells with either A549 or K562 cells. For that I am going to stain NK cells with lysotracker, cancer cells with membrane stain, and then add a dead cell stain in the medium to visualize the real-time killing of cancer cells by NK cells.
In regards to K562 cells, which are suspension cells, I found a protocol that uses an antibody (mouse-antihuman glycophorin A) to bind K562 to the wells.
My question is, for folks that have done live-cell imaging of NK cells with any other suspension cell, what other type of surface coating could work besides that antibody?
Thanks in advance.
Live-imaging of natural killer (NK) cells allows for the real-time visualization of their dynamic behaviors, interactions with target cells, and migratory patterns. This technique provides valuable insights into NK cell biology, including their cytotoxic activity and immunoregulatory functions. Here's a general overview of how live-imaging of NK cells can be performed:
Overall, live-imaging of NK cells provides a powerful tool for studying their dynamic behavior and functional responses in real-time, contributing to our understanding of NK cell biology and immunotherapy strategies.
- Badges
- Science topic
- Similar topics
- Medicine
- Disease
- Infectious Diseases