Is there a way to sufficiently slow BODIPY 581/591 reaction progression when I have a lot of samples to run on flow?

31 October 2023 1 10K Report

I am trying to use BODIPY on cultured B cells and then analyze using flow cytometry. However, I will have close to 80 samples in a 96 well plate and am afraid that the fluorescence shift will be significantly different as a function of when the samples are run (i.e. more shift in the later wells). I have been looking for ways to slow or halt the reaction since BODIPY is not fixable, but have not found much. Any advice from those familiar with BODIPY protocols? Thanks!

Adam B Shapiro

I'm not familiar with the protocol, but my suggestion is to make additions of BODIPY to the wells at time intervals corresponding to the amount of time it takes to analyze the samples, so that all samples are treated with BODIPY for the same length of time.

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