Hi all,

I am trying to purify ribosomal proteins. Does anyone happen to have a protocol?

The first step is isolation of ribosomes, then extraction of rProteins.

It looks like that the most common way to isolate ribosomes is Sucrose Gradient Ultracentrifugation which requires unltracentrifuge. I am wondering is there a simpler method to isolate crude ribosomes? The purity of ribosomes does not need to high.

For the extraction of rProteion, will urea buffer or SDS buffer work? The structure and activity of rProteins does not need to be maintained during this step.

Thank you all for your kind help.