Hi there,

I am doing regular confocal experiments to observe my over-expression target protein localization. (which is novel, encoded by a novel transcript, that is all I can say).

My OE protein has mcherry tag at its C-terminus, and I am co-staining with some well-known subcellular organelles such as: ER (PDI), mitochondria (mitotracker) and lysosome (LAMP1).

I just want to gain some evidence from the co-staining results which may provide some clue about my novel protein functions. I found: (1) my protein formed foci in cytosol; (2) my protein has very high co-localization efficiency with LAMP1 signals.

What do these mean? My protein also has a C-terminus IDR which even predict the overall structure using Alphafold, it didn't give very clear structure elements such as α-helix or sheets.

Anyone who is familiar with lysosome functions, biogenesis or metabolism, could you please provide some information or interpretation with above information? One question I want to ask is, how can I know if my protein functions in lysosome, or just being dragged into lysosome due to misfolding?

Thank you all so so so much!