Congcong Lu

4 Questions 7 Answers 0 Followers

Questions related from Congcong Lu

Is there a way to isolate ribosomes without using Sucrose Gradient Ultracentrifugation?

Hi all, I am trying to purify ribosomal proteins. Does anyone happen to have a protocol? The first step is isolation of ribosomes, then extraction of rProteins. It looks like that the most...

20 August 2022 3,277 0 View

Does anyone know a software could analyse photo-crosslinked MS/MS data?

Dear all, I am trying to locate the protein reacting sites after SBED modification. Generally, protein A was firstly modified by SBED through NHS/NH2. Then, incubated with protein B and irradiated...

10 August 2016 6,859 0 View

THPTA and TBTA, which one is better to be a Cu(I)-stabilizing ligand in click chemistry?

THPTA and TBTA are two most common used Cu(I)-stabilizing ligands. Besides the solubility, is there any difference in efficiency? How long will CuSO4/THPTA be stable when the solution is frozen?

06 November 2014 4,712 10 View

How can I destroy non-covalent interactions between proteins?

NaCl could destroy the electrostatic interactions. HAc could damage the hydrogen bond between proteins. Is this right? How to destroy non-covalent interactions between proteins?

05 August 2014 8,279 4 View