Dear colleagues:
I have been trying to establish a few HP-TLC methods in the lab. In general, for quantification purposes, I prefer CuSO4+acid charring, to primuline staining. Lately I have been trying to isolate cardiolipin (from other lipids such as PA, which loves co-eluting with it) and the best solution so far in my system is a mix that contains triethylamine (TEA). However, CuSO4 charring does not work in its presence (and seems not be the only one) and lately primuline is giving me uneven fluorescence (bright ellipse and dark borders), which is unexpected.
I am using silica 60 plates without F254.
Is there a way to get rid of TEA in order to do a proper staining?
Thank you.
My Friend , can see the links below and file attach if can give you answer for question
https://chem.libretexts.org/Ancillary_Materials/Demos_Techniques_and_Experiments/General_Lab_Techniques/Thin_Layer_Chromatography
https://www.orgchemboulder.com/Technique/Procedures/TLC/TLC.shtml
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