A. Rita D. Araújo

4 Questions 15 Answers 0 Followers

Questions related from A. Rita D. Araújo

Is there a way to get rid of TEA in a TLC plate in order to do proper staining?

Dear colleagues: I have been trying to establish a few HP-TLC methods in the lab. In general, for quantification purposes, I prefer CuSO4+acid charring, to primuline staining. Lately I have been...

21 January 2021 9,249 1 View

Heat and plastic in MTBE lipid extraction?

When extracting lipids with the MTBE method, the indicated temperature is RT. In addition, drying the samples in a speedvac is mandatory due to the presence of water and such procedure is long and...

15 October 2020 9,935 2 View

Why do I have two bands of yeast mitochondria in a Percoll gradient?

Mitochondria from S. cerevisiae cells in early stat phase were isolated using a percoll gradient. As far as I know it is expected that one brownish band will appear in the gradient. However, there...

06 January 2015 4,628 3 View

Isolation of mitochondria from yeast using lyticase and a teflon or glass pestle. Cell walls don't break. Any suggestions?

I tried to isolate mitochondria from yeast, following a protocol available in jove.com, by Christopher Gregg. However I only had access to lyticase, not zymolyase (which seems to be better in...

22 August 2014 9,060 17 View