A. Rita D. Araújo

6 Questions 19 Answers 0 Followers

Questions related from A. Rita D. Araújo

Where/how should molten phosphoric acid be stored?

Dear all, last september I acquired solid orto-phosphoric acid in a HDPE container. I came to realize that it is mostly liquid now, I suppose molten.. I was wondering: 1 - should I keep it in the...

20 May 2021 9,188 3 View

Is there a way to get rid of TEA in a TLC plate in order to do proper staining?

Dear colleagues: I have been trying to establish a few HP-TLC methods in the lab. In general, for quantification purposes, I prefer CuSO4+acid charring, to primuline staining. Lately I have been...

21 January 2021 9,450 1 View

Heat and plastic in MTBE lipid extraction?

When extracting lipids with the MTBE method, the indicated temperature is RT. In addition, drying the samples in a speedvac is mandatory due to the presence of water and such procedure is long and...

15 October 2020 10,029 2 View

Which reagent-acid pair for lipid TLC?

I have been looking for information on lipid quantification in TLC plates. I would like to use malachite green but protocols pair it with perchloric acid. Using the latter is not an option at the...

21 August 2020 5,283 4 View

Why do I have two bands of yeast mitochondria in a Percoll gradient?

Mitochondria from S. cerevisiae cells in early stat phase were isolated using a percoll gradient. As far as I know it is expected that one brownish band will appear in the gradient. However, there...

06 January 2015 4,678 3 View

Isolation of mitochondria from yeast using lyticase and a teflon or glass pestle. Cell walls don't break. Any suggestions?

I tried to isolate mitochondria from yeast, following a protocol available in jove.com, by Christopher Gregg. However I only had access to lyticase, not zymolyase (which seems to be better in...

22 August 2014 9,124 17 View