6 Questions 19 Answers 0 Followers
Questions related from A. Rita D. Araújo
Dear all, last september I acquired solid orto-phosphoric acid in a HDPE container. I came to realize that it is mostly liquid now, I suppose molten.. I was wondering: 1 - should I keep it in the...
20 May 2021 9,184 3 View
Dear colleagues: I have been trying to establish a few HP-TLC methods in the lab. In general, for quantification purposes, I prefer CuSO4+acid charring, to primuline staining. Lately I have been...
21 January 2021 9,434 1 View
When extracting lipids with the MTBE method, the indicated temperature is RT. In addition, drying the samples in a speedvac is mandatory due to the presence of water and such procedure is long and...
15 October 2020 10,023 2 View
I have been looking for information on lipid quantification in TLC plates. I would like to use malachite green but protocols pair it with perchloric acid. Using the latter is not an option at the...
21 August 2020 5,278 4 View
Mitochondria from S. cerevisiae cells in early stat phase were isolated using a percoll gradient. As far as I know it is expected that one brownish band will appear in the gradient. However, there...
06 January 2015 4,675 3 View
I tried to isolate mitochondria from yeast, following a protocol available in jove.com, by Christopher Gregg. However I only had access to lyticase, not zymolyase (which seems to be better in...
22 August 2014 9,117 17 View