Is there a way to distinguish concatameric DNA from circular episomal DNA in a cell population containing actively replicating herpesvirus?

Lena Nicole Lupey-Green @Lena-Lupey-Green

21 May 2015 1 8K Report

In a cell population in which EBV is undergoing lytic replication, is there a way to distinctly measure concatameric DNA and episomal DNA?

Lena Nicole Lupey-Green

Yes, Yijie, I am referring to multiple copies of the EBV genome forming concatamers via rolling circle replication. Based on your description, it seems like this method should be able to distinguish between the linear concatameric copies and the episomal copies.

http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC255605&blobtype=pdf

This seems to be exactly what I am trying to accomplish. I was hoping to find a way to quantify the linear vs circular DNA by qPCR, but it seems this may be the most feasible option

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