Hi Vaibav,

If you elaborate your question, what cell lines and what volume you are planning to get protein from would be better.

For research purpose, if you are using a 6-well plate you can add eGFP as a reporter in one of the wells and that can give you an idea about the success of transfection as well as transfection efficiency. 

I don't think so this is very quick way but it can give you some idea  24 hrs post transfection

Good Luck

Cheers

Either you check by Fluorescent M or Reverse transcriptase PCR.

Hi Vaibhav,

You can either use

1) GFP reporter in one of the plasmid

2) use ELISA to detect viral protein p24 in supernatant after 24,48hr

3) if vector has selection marker, add drug such puromycin after 24hr see resistance..

Good luck

Anand