Vaibhav Murthy

3 Questions 2 Answers 0 Followers

Questions related from Vaibhav Murthy

Can't figure out why my bands appear at a different height?

I have been having an issue with my western blots where the bands appear at a completely different size. I have probed for these proteins in the past with success and the same samples have been...

27 July 2021 660 3 View

What is the actual function of a Pre-protrypsin Leader sequence?

I have a plasmid that has a N-terminal FLAG-tagged protein preceded by a pre-protrypsin leader. The WT protein has a GPI anchor, that presumably is intact here. I looked on Milipore, and it says...

27 June 2019 2,537 0 View

Is there a way to check for tranfection and retrovirus production?

Hi, I am trying to make retrovirus with the following: Packaging plasmids: pJK3, CMV-Tat, Vsv-g Transfer plasmid: pLXSN Is there a quick way to see if the transfection was successful and that...

05 October 2017 6,588 3 View