Macrophages and also THP-1 cells produce ApoE. I would like to avoid using recombinant ApoE, therefore I am thinking of isolating it from macrophage or THP1 cell culture supernatants, using HPLC maybe? I would be grateful for any suggestions.
Lots of people have used heparin sepharose to isolate apo e. Look through these papers to find the best conditions for you.
http://scholar.google.com/scholar?q=apoe+%22heparin+sepharose%22&btnG=&hl=en&as_sdt=1%2C21
I will look it up. Many thanks!
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