Elwira Kaminska

4 Questions 9 Answers 0 Followers

Questions related from Elwira Kaminska

The most common melanoma cell lines?

I am looking at cytokine profile in supernatants from various cancer cell lines. I would like to have a couple of cell lines from particular type of cancer, e.g. pancreatic, glioma, leukemia. At...

07 July 2013 9,219 3 View

Positive signal in ELISA in the absence of coating antibody?

I am trying to optimize an ELISA for ApoE3 protein. I have paired antibodies and am trying to find the best concentration for the ELISA. I have experienced that with high concentration of...

05 May 2013 4,405 5 View

Is there a way of isolating and purifying apolipoprotein E (ApoE) from cell culture supernatants?

Macrophages and also THP-1 cells produce ApoE. I would like to avoid using recombinant ApoE, therefore I am thinking of isolating it from macrophage or THP1 cell culture supernatants, using HPLC...

02 February 2013 9,117 2 View

Do you know a good quality control test for the biological activity of native human ApoE derived from serum?

I am using native human ApoE derived from serum for cell culture studies. The company that I have ordered it from is not able to tell whether this protein is biologically active or not, because it...

11 November 2012 7,405 5 View