Is there a valid, maybe even better method/device to characterize cells upon markers and morphorphology besides flow cytometry/FACS?

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Has anyone use Emx1Cre adult mice to label pyramidal neurons?

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OMVs labeling for uptake assay?

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Problem with F-AAS Zinc quality controls standards?

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Does anyone has a CamKII primary antibody to recommend for immunostaining in slices?

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How to CRISPR-KO and CRISPR-HDR of p53 in fibroblasts?

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ImageJ or QuPath?

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Which method/scale should I use to measure self-disclosure online?

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What are the emerging trends and opportunities for STEM education and careers in the online space?

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Seeking Advice on Viability and Execution of Undergraduate Thesis Topic?

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Who will be moral responsible for the death of thousands of people in the event of an earthquake?

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Is there any way to quantify bacterial and fungal cells in their mixed culture?

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What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

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Why activated CAR-Jurkat cell could not kill targets?

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Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

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How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

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Blake Rust

Fluorescent microscopy?

Magdalena Schimke

microscopy has other issues that we want to overcome. standardly, people use facs/cytometers to do so... but mostly for stem cells this harbors other troubles.

thanks regina, i have a look. btw also our agilent bioanalzyer 2100 has options to upgrade but they are expensive and not yet well established

Lukas Peintner

Hi Magda,

I think the products of http://chemometec.com/ are doing a good job. But if you want to be 100% positive about your cells there is basically no alternative to FACS.

cheers