Is there a some way that can be used for normalising cytokines if I don't have a cell lysates?

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David Todd, Experimental organic chemistry, Prentice-Hall, Englewood Cliffs, NJ, 1979. 347 pp. ?

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To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

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Amanda Eakin

I think the cheapest/quickest way would be to do a duplicate plate and perform only cell counts/viability on this. Then normalize your main plate data using the counts/viability. Maybe you already have this info from prelim experiments?

Shen-An Hwang

Since you don't have the cell lysates anymore... you MIGHT be able to normalize to total secreted protein. But you should really, as suggested above, repeat your experiment and normalize to cell count.