Denis Komarov

5 Questions 17 Answers 0 Followers

Questions related from Denis Komarov

How to normalize a supernatant(media) in western blot?

Hi, I want to quantify the amount of collagen(s) that got secreted outside of cells to ECM through Western Blotting the media, however I'm not sure which housekeeping gene I can use in order to...

28 April 2023 2,130 4 View

What repeated measures statistical tests should I use with uneven sample size and parametrical/nonparametrical distribution?

I'm looking for a way to measure significance + variance homogeneity/heterogenity First of all I tested my data for normality and it's not always parametrical, so I think that I should use...

29 June 2021 9,498 3 View

Is there a some way that can be used for normalising cytokines if I don't have a cell lysates?

So I'm want to perfrorm an ELISA for cytokines measure (like IL-6 and IL-1), after 0/6/12/24/48/72 hours after irradiation. Problem is, that number of cells after 24H is significantly different...

05 February 2019 7,148 2 View

Homogenization of mouse brain tissues for perforoming Myelin Basic Protein (MBP) ELISA?

Does anyone know the best way to get a homogenate of mouse brain? Can I do it just by using cold PBS and a few freeze-thaw cycles?

30 October 2018 3,644 1 View

Can I use CellTiter Glo 2.0 from Promega with PBS or it's media-only?

I just really don't know why my results isn't equal when I use CellTiter-Glo and CellTiter-Glo 2. Can someone explain it to me?

16 January 2018 3,978 1 View