Dear all,
I am playing around with some new methods for bacterial expression and I want to use a model protein for which expression optimization will make the most sense.
I looked at somatotropin and endostatin, for example. But are there other small proteins which might be interesting in this context?
Awaiting your suggestions,
Regards,
Peter
Hi Peter, maybe this database will be of help.
http://crdd.osdd.net/raghava/cancerppd/
Dear Peter,
Human calmodulin (CaM1) was the one of the most ugly one proteins in my hands, when produced in bacteria due to it's degradation. Check the prices of CaM at commercial supliers - is expensive like a hell - best prove for me it makes sense to optimize its expression.
Regards - Jan
You could try free beta subunit of hCG (around 23kD) most recombinant preps have gone from the marketplace.
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