Can anyone report on the molecular weight of Q5 Polymerase?

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Amy Johnson

I seem to see more extra DNA bands using Q5 than when using phusion. Q5 is a smaller protein than Taq, I have read that.

Ajith Kumar

Hi,

Possibly due to the DNA binding protein fusion.

"Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance. "

https://international.neb.com/products/pcr-qpcr-and-amplification-technologies/q5-high-fidelity-dna-polymerases/q5-high-fidelity-dna-polymerases

Peter Rehbein

To clarify, we observe additional bands in PROTEIN gels and would like to verify whether one of These bands is Q5. Maybe anyone has already determined the molecular weight of Q5, so we could rule out that the contamination is due to Q5?