KHMgE buffer stability?

I'm putting together cell unroofing protocols based on a few papers. Unfortunately, there is no mention of whether it's ok for me to keep the KHMgE buffer in the fridge? AEBSF is usually stored at...

31 December 2018 8,486 3 View

Are there any papers or other information on the effect of different concentrations of dextran solution on cell buoyancy?

08 September 2015 2,490 1 View

How would I measure efficiency of transfection with 70kDa fluorescently labelled dextran and viability for 3T3 cells without having to use FACS?

I have limited access to the FACS and was hoping there is a way to do both tests in as little time as possible to avoid loosing viability. I will eventually move on to more sensitive cells so time...

05 June 2015 3,442 1 View

Reliability of a COMSOL model of a CVD

Hi! Mostly for those who have attempted one before: how reliable are COMSOL models of laminar flow and heat transfer in a CVD? There's obviously plenty of parameters, particularly at a molecular...

01 January 1970 6,686 5 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View