Veronika Cencen

5 Questions 6 Answers 0 Followers

Questions related from Veronika Cencen

KHMgE buffer stability?

I'm putting together cell unroofing protocols based on a few papers. Unfortunately, there is no mention of whether it's ok for me to keep the KHMgE buffer in the fridge? AEBSF is usually stored at...

01 January 2019 8,533 3 View

Is there a reliable vendor of glutaraldehyde in powder form?

For cell fixation; Thus far I've only found solution form

12 December 2018 284 2 View

Are there any papers or other information on the effect of different concentrations of dextran solution on cell buoyancy?

For example, I have a 8.603059e15 molecules of 70kDa dextran per mL of media into which I put HeLa or 3T3 cells, usually at either 1:1 or 1:3 ratio, trying to have them semi-buoyant... I was...

09 September 2015 2,553 1 View

How would I measure efficiency of transfection with 70kDa fluorescently labelled dextran and viability for 3T3 cells without having to use FACS?

I have limited access to the FACS and was hoping there is a way to do both tests in as little time as possible to avoid loosing viability. I will eventually move on to more sensitive cells so time...

06 June 2015 3,476 1 View

Reliability of a COMSOL model of a CVD

Hi! Mostly for those who have attempted one before: how reliable are COMSOL models of laminar flow and heat transfer in a CVD? There's obviously plenty of parameters, particularly at a molecular...

01 January 1970 6,720 5 View