Is there a relation between transit time and excitation laser power?

Roobala Chelladurai @Roobala-Chelladurai

17 November 2015 1 2K Report

I performed a FCS experiment on DMPC bilayer tagged with BODIPY C12 HPC.

When I increase the power of excitation laser, I observe reduction in transit times.(see Fig 1) It is effectively like I am attaining smaller and smaller PSFs with increase in excitation laser power. We are using a LEICA TCS SP5 II microscope where power can be increased/decreased by adjusting AOTF.

I am not able to understand the reason behind this trend. Is it any form of artifact? Please give your comments.

Roobala Chelladurai

Thank you Dr.Peter Kapusta for your comment.

If there is bleaching, intensity versus time plot should show me that as a decay in counts. But I do not see such decay.

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