Roobala Chelladurai

8 Questions 16 Answers 0 Followers

Questions related from Roobala Chelladurai

Are there any specific sub-phase conditions to be maintained in case of DPPG monolayer?

I am facing some issue with preparation of DPPG supported monolayer and bilayers using Langmuir Blodgett technique. They are not getting transferred efficiently as opposed to PC lipids. We also...

18 April 2018 2,064 1 View

How to calculate mean molecular area(MMA) for binary / ternary surfactants in Langmuir trough?

We are using KSV NIMA Langmuir trough for forming Langmuir monolayers. If we can input the molecular weight(M.W), volume(v) of solution and corresponding concentration(c), MMA is calculated from...

03 April 2017 4,781 3 View

Finding some strange oscillations in FCS correlation curve?

Hi, In some of our 2D FCS measurement, I am finding that the correaltion curves are showing oscillation trends towards decay. (image attached) This is not a common observation is all the...

10 March 2017 9,755 5 View

Fluoroscence quenching in supported lipid bilayers?

For some reason, recently we are facing some quenching problem in our tagged SLBs. For the same conditions(dye concentration, laser power etc.,) we have used before, intensity from the bilayer...

18 February 2016 5,997 2 View

Is there a relation between transit time and excitation laser power?

I performed a FCS experiment on DMPC bilayer tagged with BODIPY C12 HPC. When I increase the power of excitation laser, I observe reduction in transit times.(see Fig 1) It is effectively like I am...

17 November 2015 2,432 1 View

Can anyone recommend fluorescent lipid analogs?

We are looking for some STED compatible dye to use in our lipid bilayer.(Depletion laser - 592nm). Any fluoroscent lipids with excitation other than 488nm would be very helpful for our experiments.

06 April 2015 245 7 View

Does anyone know about FCS experiments on pure DPPC bilayers?

I tried some FCS experiments on DPPC bilayer(bodipy c12hpc - 10^-4 mol%). But the intensity is just decaying over t ( time trace).Is it that gel phase of DPPC is a reason? I am preparing these...

06 April 2015 4,290 3 View

Is what I get an artifact in liquid AFM image analysis?

We are working on DMPC lipid bilayers. When imaged under liquid, we observe lot of defect features. As the bilayer is prepared using Langmuir Blodgett, we anticipate some defects. But how do I...

23 January 2015 9,689 16 View