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Questions related from Roobala Chelladurai
I am facing some issue with preparation of DPPG supported monolayer and bilayers using Langmuir Blodgett technique. They are not getting transferred efficiently as opposed to PC lipids. We also...
18 April 2018 2,082 1 View
18 April 2018 5,447 0 View
We are using KSV NIMA Langmuir trough for forming Langmuir monolayers. If we can input the molecular weight(M.W), volume(v) of solution and corresponding concentration(c), MMA is calculated from...
03 April 2017 4,791 3 View
Hi, In some of our 2D FCS measurement, I am finding that the correaltion curves are showing oscillation trends towards decay. (image attached) This is not a common observation is all the...
10 March 2017 9,767 5 View
We are planning to study this in supported lipid bilayer platform. We will introduce molecules(tagged with dye B) to the lipid bilayer(tagged with dye A). Both dye A and B are spectrally...
10 January 2017 5,557 3 View
For some reason, recently we are facing some quenching problem in our tagged SLBs. For the same conditions(dye concentration, laser power etc.,) we have used before, intensity from the bilayer...
18 February 2016 6,003 2 View
I performed a FCS experiment on DMPC bilayer tagged with BODIPY C12 HPC. When I increase the power of excitation laser, I observe reduction in transit times.(see Fig 1) It is effectively like I am...
17 November 2015 2,437 1 View
We are looking for some STED compatible dye to use in our lipid bilayer.(Depletion laser - 592nm). Any fluoroscent lipids with excitation other than 488nm would be very helpful for our experiments.
06 April 2015 250 7 View
I tried some FCS experiments on DPPC bilayer(bodipy c12hpc - 10^-4 mol%). But the intensity is just decaying over t ( time trace).Is it that gel phase of DPPC is a reason? I am preparing these...
06 April 2015 4,296 3 View
I have done contact mode liquid imaging. Topographical and lateral force images are obtained in this. Can I use this to convert and obtain a friction map?
21 March 2015 2,537 3 View
Is there any recommended standard samples for performing tracking measurements (2D)? Before going to any complicated measurements, we want to run some known samples.
05 March 2015 5,820 3 View
We are working on DMPC lipid bilayers. When imaged under liquid, we observe lot of defect features. As the bilayer is prepared using Langmuir Blodgett, we anticipate some defects. But how do I...
23 January 2015 9,694 16 View
