I am working with yeast cells and want to visualise vacuoles in yeast after staining it with lysotracker red of life technologies as i have lysotracker red only currently as a lysosomal marker. ordering reagent will take time and my experiment is stuck :(
Hi Pratima,
I have used it once and followed the following protocol link below (follow method for suspension cells). I worked really well.
https://tools.thermofisher.com/content/sfs/manuals/mp07525.pdf
Alternatively, recently I came across this new article, where the authors claim that Cresyl violet, is way much more better than any lysosomal probe (lysotracker etc) for visualizing lysosomes in Yeast cells. I suggest you can contact them and ask for a sample for a start.
http://onlinelibrary.wiley.com/doi/10.1111/tra.12447/full
Hope am helpful.
Good luck.
cheers
Article Cresyl violet: A superior fluorescent lysosomal marker
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