Is there a protocol to purify poly-clonal antibodies using E.coli lysate?

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01 September 2023 3,874 2 View

Thank my audience ?

I would like to thank my audience for reaching my target level in one month as a newbie. Please stay with me. I will make amazing discoveries for you. Thank you for your support and motivation....

22 July 2023 9,113 2 View

Global issues and solutions ?

Most respondents to the 2022-2023 Global Risks Perception Survey chose “Energy supply crisis”; “Cost-of-living crisis”; “Rising inflation”; “Food supply crisis” and “Cyberattacks on critical...

21 July 2023 8,978 2 View

What are the importance of research interest score ? Will it important for scholarships or higher education ? What are the benefits?

18 July 2023 4,902 0 View

How could I assess "Academic performances" in target college students without their examination marks? ?

I need to measure the relationship between mobile phone addiction and academic performance

28 February 2023 1,009 1 View

Can anyone explain how to export a dendrogram constructed using DARWIN software with unit identifiers?

I'm trying to export a large tree constructed for 2018 species using 10,000 DArT markers using DARWIN. I can export this tree as a Nexus/Paup, but only without unit identifiers.

23 January 2023 4,917 0 View

How to simulate thermoelectric cooler model in COMSOL?

I need an explanation of how to add a study of "Coefficient Of Performance(COP)" into the thermoelectric cooler model. I have attached my current working file below.

22 September 2021 620 2 View

What is the best delivery method (subcute or ip) to inject ampethetamine and dizocilpine?

I want to know what is the best administration method of amphetamine and dizocilpine in mice. Specifically, intraperitoneal or subcutaneous injection before behavior tests.

26 February 2019 2,086 0 View

Do any one knows about Non linear modeling techniques for behavioral modelling?

I'm exploring non linear modelling technique for demand forecasting in transportation sector where alternative transport mode implement. As a start it is good to have some ideas from you Thank you

18 October 2018 7,171 1 View

Has anyone worked with 5KC T-cell Hybridoma (Mouse) cell line before?

I am using 5KC T-cell Hybridoma cell line in one of my T-cell receptor (TCR) studies and was wondering whether anyone have more information about their immunological characteristics. Like what...

27 September 2018 1,650 0 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

Which distribution type should I use when calculating the average particle size from TEM image? and how to calculate the error ?

average particle size calculation from TEM

04 August 2024 2,921 1 View

Is it necessary to covary exogenous constructs in a structural model?

I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...

03 August 2024 6,028 4 View

In SEM, if a latent variable consists of three observed variables, and each observed variable has four items, what should I do?

Suppose I am studying the construct of "Academic Motivation" (latent variable) in students, which is measured through three observed variables: "Intrinsic Motivation," "Extrinsic Motivation," and...

31 July 2024 3,913 6 View