I want to know what is the best administration method of amphetamine and dizocilpine in mice. Specifically, intraperitoneal or subcutaneous injection before behavior tests.
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01 September 2023 3,874 2 View
I need to measure the relationship between mobile phone addiction and academic performance
28 February 2023 1,009 1 View
I'm exploring non linear modelling technique for demand forecasting in transportation sector where alternative transport mode implement. As a start it is good to have some ideas from you Thank you
18 October 2018 7,171 1 View
I am using 5KC T-cell Hybridoma cell line in one of my T-cell receptor (TCR) studies and was wondering whether anyone have more information about their immunological characteristics. Like what...
27 September 2018 1,650 0 View
I am looking to analyze how the solubility of aggregation-prone proteins (like Tau, A-beta or a-synuclein) behave under different protein disaggregases and chaperons. Something along the line of...
13 September 2018 8,405 1 View
The Public transpiration system of all developing countries are getting popular because of the integrated transportation development projects. Having practice a different kind of technology with...
12 February 2018 5,136 6 View
Currently I have to do a site plan in my academic works. I like to do a site plan for a critical junction area of Galle town, Sri Lanka. From the site planning I like to overcome the traffic jam...
29 January 2018 8,498 4 View
TIA comes with the legal background. Therefore it has been allowed normalize traffic level for all. But there are significant road segments that cannot allow the legal level. If there any...
06 January 2018 3,105 2 View
Damaging their genital spines confuse the identification between Sergentomia and Phlebotomus genera.
15 August 2017 672 0 View
I'm using amber suppressor tRNA in E.coli to perform in vivo photo-cross-linking experiments to study certain protein-protein interactions at the outer membrane. I do get indications of...
13 October 2016 6,316 5 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View