Dilshan Gunasinghe

5 Questions 10 Answers 0 Followers

Questions related from Dilshan Gunasinghe

Has anyone worked with 5KC T-cell Hybridoma (Mouse) cell line before?

I am using 5KC T-cell Hybridoma cell line in one of my T-cell receptor (TCR) studies and was wondering whether anyone have more information about their immunological characteristics. Like what...

28 September 2018 1,696 0 View

How to locate Hypervariable regions (complementary determining regions i.e. CDRs) for a given TCR crystal structure?

I looking at TCR-pMHC crystal structures and would like to locate CDR1, CDR2 and CDR3 regions in alpha and beta subunits of TCR. Is there an online predictor or a database for this?

13 June 2018 7,040 2 View

How to enhance your in vivo photo-cross-linking experiments in E.coli?

I'm using amber suppressor tRNA in E.coli to perform in vivo photo-cross-linking experiments to study certain protein-protein interactions at the outer membrane. I do get indications of...

14 October 2016 6,354 5 View

Does anyone has any expertise in the applications of Ripley's (K) and (L) functions for Cluster analysis purposes?

I am new to Cluster analysis, and I’m using a spatial descriptive statistical method called the Ripley’s (K) function for my analysis purposes. I read some really good papers which explained the...

14 November 2014 8,139 2 View

Is there a protocol to purify poly-clonal antibodies using E.coli lysate?

I already have an Affinity purified antibody which works well in Western Blots, but it shows non specific binding when I'm using this for Immunofluorescence applications.

25 June 2014 9,873 2 View