Testing sensitivity to antibiotics may vary according to type of bacteria, type of antibiotic and the medium in which bacteria exists (saliva, gastric, etc)
You have to follow CLSI.
At first step isolate your bacteria from your sample and then choice your options for antibiogram such disk diffusion.
you can do antibiotic sensitivity test for bacteria also with MIC test not only by discs and the results will be better than discs if you do it with microtiter plate
Hi
Article Pharmacological synergism of bee venom and melittin with ant...
Isolate bacteria and use pure culture pick two to three colonies and put them in Muller Hinton broth until you reach 0.5 McFarland then separate it on Muller Hinton agar plate by sterile swab , put antibiotic discs like amoxicillin and Clindamycin ( Kirby Bauer disc diffusion method ) after incubation measure the diameter and you will know if the bacteria is resistant or susceptible from NCCLS guidelines
Rawan F Abu-Zineh
Are you sure for preparation 0.5 McFarland in disk diffusion method use BHI broth?
Do you have any references?
see these sites and papers
- https://academic.oup.com/cid/article/58/8/1140/355853
- http://www.ijmm.org/documents/Antimicrobial.doc
Article Comparative Efficacies of Amoxicillin, Clindamycin, and Moxi...
- https://emedicine.medscape.com/article/228936-medication
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