Hi all, I'm trying to extract total RNA from exosomes using RNAeasy Qiagen. I've tried to use 2x109 EVs, as I found on a paper, but it didn't work. When we measure on Nanodrop, the concentration is 0 ug/ul. I know that Nanodrop could not measure it, so when I used total RNA to make cDNA and ran a gel, I still could see nothing. I probably need more RNA. So I`d like to know how many EVs you use to extract total RNA and make cDNA.