Hello,
I'm interested in testing my compound to see if it inhibits/activates enzymes, multiple enzymes at the same time. Is it something unheard of or is there such a technique/method? Thank you very much.
The simplest approach is to run separate assays for each enzyme.
Another angle on this question is that compounds that inhibit multiple enzymes are frequently non-specific inhibitors (sometimes referred to as PAINs).
https://chembiohub.ox.ac.uk/blog/2015/03/10/pains.html
If you have a mixture of these enzymes, I'm assuming much more than 2, and add an inhibitor/activator compound and then want to know which enzyme is affected won't you have to assay each enzyme separately anyway? Do these enzymes act on different substrates? Aren't you going to have to deal with degrees of inhibition/activation for each enzyme and therefore have an extremely complicated kinetic analysis for the mixture?
Previous answers are correct ones. The sole point I would like to add is that it is possible to perform in a single assay (experiment) not in a single test tube. In this case you may have sets of different enzyme mixtures with different levels of substrates for each enzyme ans you run +/- putative inhibitor in time order with adequate stop for each enzyme activity. Preserve (constant) the incubation time for each sample and you will get a preliminary answer for all the enzymes on a single assay.
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