Lana Segizbayeva

5 Questions 5 Answers 0 Followers

Questions related from Lana Segizbayeva

Do you know a good protocol for purification of long (125 nt) biotinylated oligos for MS experiment?

Hello, Could you recommend a good protocol for purification of 5' biotinylated oligos that will further be used in a MS experiment (need to avoid EDTA, glycerol and other chemical contaminants)?...

16 May 2018 2,378 4 View

How to find a sequence in the BAM file (Integrative Genome Browser)?

Hi I have a bam file for an organism. I need to find all the places this sequence is found in the bam file. Is there a way to pull these kind of data from bam file used for IGV instead of zooming...

06 December 2017 1,105 4 View

Which is a better microscope for imaging yeast mitochondria, also to measure FRAP - Olympus FV1000 MPE, Leica SP8 or spinning disk?

I'm trying to get better pictures of yeast mitochondria and the facility I have access to has a choice of spinning disk, Olympus FV1000 MPE, Olympus FV1000 - two photon, and Leica SP8 MP. I think...

04 April 2017 7,688 2 View

What is the most accurate method to measure yeast MnSOD?

I need to measure the MnSOD enzyme activity in yeast mitochondria. I know that that the IMS contains some Cu/Zn isozyme but I am interested in the MnSOD (which is in the mit. matrix). I have not...

13 September 2016 4,286 2 View

Is there a method to find out whether my compound inhibits/activates enzymes (different) enzymes at the same time? Some kind of tandem screening?

Hello, I'm interested in testing my compound to see if it inhibits/activates enzymes, multiple enzymes at the same time. Is it something unheard of or is there such a technique/method? Thank you...

26 April 2016 5,343 3 View