I have a couple chloroplast genomes deposited in GenBank where IR sequences are non-identical, but they should be. They might be sequencing and/or assembly errors. Is there a way to estimate this from the deposited genome sequences?
Unless you have the raw data with fastq files, you can't do that. Which kind of data do you have?
Please may I ask if non-salaried Public Contributors conduct data collection interviews or Focus Groups with NHS patients as part of your study? This means that the Public Contributor will either...
29 July 2024 3,085 0 View
Hi everyone, I am currently working on RNA extraction on tissue, but unfortunately, I only have large frozen clinical tissue on hand. In order to maximize the RNA integrity, I plan to use...
25 June 2024 7,550 1 View
i want to write literature review on the topic " production of 3d PLA filament"
19 June 2024 1,712 2 View
Hi, I am currently working on RNA extraction on blood serum samples using Trizol. However, the RNA yield is quite bad, on average, only yielded 200ng per 200ul of sample input, and the A260/280...
18 June 2024 6,854 3 View
In my qualitative study, I am trying to avoid the traditional belief that types of qualitative research design are just grounded theory, ethnography, phenomenology, case study, historical, and...
15 June 2024 3,884 1 View
I have a set-up were I have a bottle with water (DI water, 0.8uS/cm). To that bottle I have added some MgO nanopowder, at a concentration of ~ 0.08mg/ml. It appears that the white powder is...
04 June 2024 9,789 4 View
As urbanization accelerates, the environmental toll of rapid urban growth becomes increasingly apparent. Please pitch your opinion/idea on this question as we explore innovative approaches...
02 June 2024 737 3 View
I would like to look at mitochondrial density in transverse sections of intestine. I do not have access to a TEM. I cannot really determine based on an internet search whether it is feasible to do...
02 June 2024 5,373 2 View
My profile on researchGate is not yet visible on goggle
26 May 2024 5,824 1 View
Climate change-induced shifts in precipitation patterns are reshaping the availability and quality of freshwater resources worldwide. Join us in examining the far-reaching implications of these...
19 May 2024 8,764 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...
03 August 2024 2,484 1 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...
01 August 2024 1,514 1 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View