I'm encountering difficulties when it comes to quantifying the blue-stained cells in the image I've provided. These blue-stained elements represent cells, while the remaining particles are pores originating from the membrane of 24-well inserts. My goal is to eliminate these pores and isolate the cells. Is there a method available to remove the pores and exclusively highlight the cells in the image? Any assistance with this task would be highly valued.
Subodh Sharma, Yes, using artificial neural networks (ANNs) for image processing in MATLAB, you can train a model to achieve results similar to those in machine learning image processing. I have tested this approach and it works well. A detailed explanation is provided in the following YouTube playlist, with only minor modifications required: https://www.youtube.com/watch?v=oihlyCSizvU&list=PLULfBC7YW3lwaFz6tmfS6Gw6cwljTmC9r&ab_channel=Eng-KhaledWaledF.
I have attached the results as well for comparison. I hope it helps, if you need any assistance ask your queries on my WA; https://wa.me/+923440907874. Good luck !
Hi Subodh Sharma ,
So, I was able to isolate your stained cells with Colour Threshold function in ImageJ, though it's not perfect. The red region you are seeing will be measured when we proceed further for calculations. I have attached the screenshot. I hope this helps.
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