17 Questions 9 Answers 0 Followers
Questions related from Subodh Sharma
I'm encountering difficulties when it comes to quantifying the blue-stained cells in the image I've provided. These blue-stained elements represent cells, while the remaining particles are pores...
27 September 2023 5,242 2 View
I am trying to culture human preadipocytes. After receiving a cryopreserved vial of preadipocytes, I cultured the cells in DMEF/F-12 media containing 10% FBS, 1% ultra-glutamine, and primocin....
24 November 2022 6,948 1 View
In my experiment fibroblasts (cell layer) get dettached on treatment with TGF-beta (5, 10, 15 ng/mL) with media color change. TGF-beta from Sigma was reconstituted in citric acid with pH 3.0 and...
07 May 2018 4,776 4 View
I wanted to check translocon proteins (Sec61 alpha, beta, gamma) in endoplasmic reticulm. I got band for alpha subunit protein. However I could not band for beta sub-unit (15 kDa) and gamma...
07 September 2016 7,013 2 View
I am trying to detect Sec61-gamma with protein size 7.5 kDa. Does anybody have suggestions for me on working with such small protein size? What advice you give me regarding the process?
27 July 2016 7,890 2 View
Actually I want to check the level of phospho AKT in wt and resistant PC9 following treatment with gefitinib. I tried with 24 and 48 hours gefitinib treatment and the result was, phospho AKT could...
31 March 2015 1,261 4 View
I performed invasion assay for PC9 cell and I found many way of expressing invaded cells through the matrigel in published papers: 1) simply the number of invaded cells 2) % of invaded cells and...
24 March 2015 2,022 2 View
Actually I want to perform invasion assay for PC9 cell. Please help me out, how can I perform this. What differences are there between migration assay and invasion assay method?
05 March 2015 10,076 5 View
I need to perform Gefitinib induced PC9 apoptosis using FACS. Please help me with required protocol for this. What cell number should I use in the method?
10 February 2015 2,767 2 View
I want to make a growth curve between control and test PC9 cell, in vitro. So please suggest me the method for it. Thank You in advance.
07 January 2015 589 4 View
I am looking for a good relaible and efficient software to analyse wound healing assay. I found one of them is the TScratch Assay. Please whoever is expert on this help me get the software and...
30 December 2014 5,281 3 View
I need to do migratation assay with pc 9 cell. I am not getting consistent results as I used different cell numbers in the assay. If anyone can help me if cell density in the assay does impact in...
22 December 2014 2,173 4 View
I have some problem in migration assay. In the migration assay, which cells are better to consider as migrated cells, cells on the outer surface of membrane ( transwell insert ) or the cells...
18 December 2014 7,729 5 View
If you have induced either PC9 or HCC827 cells with macrophages, then what kind of morphological changes have you seen in the cell? Does this change in morphology affect migration, wound healing...
08 December 2014 7,142 0 View
I read in papers; some have used 4% formalin and some have used 100% methanol. Further, in case of PC9 cell staining with DAPI or Hoecast 3332, how important is permeabilization step? Is the step...
24 November 2014 4,638 3 View
I want to check the migration of those PC9 cells, induced with macrophage conditioned medium. How to perform migration assay on PC9 cell using this conditioned medium?
24 November 2014 9,168 1 View
If there is any specific chemoattracant for the PC9 cell?
20 November 2014 7,673 4 View
