Is there a method for quantifying Nisin A by RP-HPLC?

09 September 2014 1 2K Report

I need to quantify Nisin by RP HPLC in a preservative product for food. Thanks.

Nisin, as monensin is a ionophore. They share some structural similarities, my guess is probably a post-column derivatization with vainillin in methanol and sulfuric acid will do the trick (a pink colored derivative is obtained for polyether compounds such as monensin, lasalocid and salinomycin and maduramicin) if you a are using VWD/DAD detection. See for instance: AOAC 997.04; J AOAC Int. 80(4):693-702. Hope this helps.

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