Looking for a method to create a solution containing dissolved aluminium and silicate but without the gel formation.
You can try to reduce water binding using any methods you known or else you can add any fromamide agent to reduce the formation.
Does anyone remember how to take a FISH image with Metasystems Isis software and then take another at a different focal plane and combine them? I used to do this but now can't remember! Thanks
14 May 2024 10,025 0 View
Has any got experience packaging MSCV vectors with large cargo? Im in the final stages of plasmid design and so far the size is ~8.8kb.. I'm concerned the virus will fail packaging.
24 April 2023 4,508 1 View
Oliver Wendel Holmes believed that justice had to do with customs that vary from one society to another, relativistic and without a foundation as an overarching principle. Marxist believe that...
22 March 2023 4,828 4 View
Recently I’ve started seeing these clumps in my primary MSC flask (T175). I first noticed these compact clumps in a flask near 80% confluence but I’m also seeing it in less confluent flasks. The...
22 February 2023 9,528 3 View
My lab has been using the Qubit ssDNA kit to quantify cDNA prior to qPCR input, but we have doubts about the accuracy of this kit. The MFR explicitly states that the Qubit ssDNA kit does not bind...
14 December 2022 1,052 2 View
It is said that the p value for a t-test can be calculated as one-tailed or two-tailed. This makes sense as Condition A scores can be higher, or lower than Condition B scores (so a two-directional...
04 March 2022 3,629 4 View
I have seen some literature discussing clearance of LPS from the bloodstream, where it can be cleared pretty well in about 6 hours (Lipopolysaccharide Is Cleared from the Circulation by Hepato......
05 August 2021 2,263 1 View
Traditionally patients in South Africa, particularly in rural areas retain their own clinical record, often in an exercise book or similar. Healthcare staff also record the history of a particular...
14 May 2021 3,930 3 View
Is it possible to have two protein variants transcribed from a transfected plasmid (or viral vector)? Not in the sense of using IRES or P2A/T2A, but rather in having two proteins with the same C...
02 March 2021 7,761 2 View
All suggestions welcome. We are curious to find out what works well, and to hear about diverse methodology. Thanks! We currently have restricted access to a lab where Silver staining etc could...
09 June 2020 9,263 3 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Is this circle one of the rings?
03 August 2024 5,988 0 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...
29 July 2024 950 4 View
How can the rubber fibres produced by the electrospinning device be removed from the fibre collector (aluminium foil) without affecting the orientation of the rubber fibres for use in reinforcing...
26 July 2024 8,281 0 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
Hello, I was running a 12% SDS Page electrophoresis on few granulosa cell samples and got this result after the ponceau staining. The total protein lysate seem to aggregate at 70 kDa ladder mark...
21 July 2024 5,128 4 View