What are typical methods of producing HEA's?

I've been curious about how exactly HEA's are manufactured. Are they predominately cast? Printed with additive manufacturing?

29 December 2019 6,887 2 View

Qiagen Blood & Tissue Kit with plants?

I know Qiagen makes plant specific extraction kits, but has anyone tried the DNeasy blood and tissue kit with plant material? Thanks, Matt

24 March 2019 8,520 2 View

Are there theoretical limits to how many viable T cells can be derived from peripheral blood donation (e.g., PBMCs) of a single healthy donor?

Immune oncology drug programs are increasingly moving towards use of allogeneic T cells which can be engineered and used with unmatched recipients. Celyad, for instance, recently got clearance...

15 August 2018 1,122 2 View

Can isoelectric points of my protein affect nickel affinity chromatography?

When purifying my his-tagged protein much of it was eluting at only 50mM imidazole. The protein was binding to the charged beads as shown by samples from the beads and supernatant after incubation...

30 March 2018 5,096 4 View

Negative % degree of hydrolysis values for OPA assay?

Hi, I am using the o-phthalaldehyde assay (OPA) to quantify the % degree of hydrolysis in bacterial fermented milk supernatants. However, for some of my isolates I am obtaining negative %DH...

22 February 2018 2,459 3 View

How long does it generally take to recieve the full-text article/journal?

I have made several requests this afternoon for full-text articles/Journals. I am a new user to this site and am just curious how long it generally takes to process those requests. Thank you!

11 December 2017 4,893 5 View

Are there any recommendations on how to clean a biochemistry laboratory dishwasher?

Our biochemistry lab received a dishwasher from an agriculture lab. We were wondering if there are any recommendations on how to clean the dishwasher before we use it ourselves.I'm assuming we...

15 March 2017 3,072 1 View

Do all Pseudomonas aeruginosa fluoresce under UV light?

17 February 2017 9,895 2 View

I'm looking for the pFLP2 plasmid?

I'm trying to get my hands on the plasmid pFLP2 for recombineering purposes. Does anyone out there have some they'd be willing to send? Or know of a lab that does? Thanks in advance.

25 September 2016 7,634 3 View

What is the 'rescue' of an inhibitory effect (in order to rule out toxicity)?

I am going over a reviewer's comments for my paper and being new to the research world I am not sure what the reviewer means when he/she is asking me whether the inhibitory effect of my product...

10 September 2016 9,255 7 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View