MSCV vector with 8.8kb insert between LTRs?

25 April 2023 1 5K Report

Has any got experience packaging MSCV vectors with large cargo? Im in the final stages of plasmid design and so far the size is ~8.8kb.. I'm concerned the virus will fail packaging.

Robert Adolf Brinzer

Cargos of >8kb result in reduced titers of virus. Consider switching over to a CPP functionalized filamentous phage system for really large cargos.

Does your plasmid have to be so big? What you got on it?

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