Our lab harvests HUVEC (human umbilical primary endothelial cells) and endothelial cells can support COVID19 growth. The mothers will be screened for Covid, but we all know that there are false negatives, particularly in very early or very late stages of infection. Does anyone know of a kit that could be used to detect contaminated cell lines?
Thanks,
Naiche
Dear Naiche!
You look at this article, please:
https://www.biorxiv.org/content/10.1101/2020.08.31.276725v1.full.pdf
Cell culture and virus infection.
Human umbilical vein endothelial cells (HUVEC) were isolated from anonymously acquired human umbilical cords according to the Declaration of Helsinki, “Ethical principles for Medical Research Involving Human Subjects” (1964)
Detection of SARS-CoV-2 by using qRT-PCR. For the determination of SARS-CoV 2, we used the QIAamp Viral RNA Mini Kit (Qiagen, Hilden, Germany) according to manufacturer's guide. A qRT-PCR from RIDAgene (r-biopharm, Darmstadt, Germany) followed on Rotor-Gene Q (Qiagen, Hilden, Germany) to detect the E-gene of SARS-CoV-2. The RNA standard curve, prepared from the positive control of the RIDAgene (r-biopharm, Darmstadt, Germany) kit, Cycle conditions were set as follows: 10 min at 58°C, 1 min at 95°C and 45 cycles of 95°C for 15 sec and 60°C for 30 sec
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