How can I dissolve decellularized ECM?

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Lukas Krasny

Hi Raizel,

8M Urea works to some extent.

Raizel Adler

Thank you Lukas Krasny

RIPA + ultrasonication worked as well

Jochen Salber

Dear Colleague,

As far as I can see, there is no single method for all the different decECMs. Depending on the species and the tissue type used or the anatomical localisation, the respective generated decECMs have different biochemical compositions. The degree of cross-linking of the different structural proteins can also be very different.

Best regards,