We are imaging what appears to be endocytosis in photoreceptors, and we want a "positve control" for what we are seeing.
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Pig slurry is rich in major and minor nutrients. Is there any way to improve / Enrich its manure quality to be used in agriculture organically ? please share your knowledge.
09 August 2024 5,605 2 View
Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...
07 August 2024 269 3 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,218 4 View
Hi How can this equation Ln(LA) = 1.038 + 0.89 ln(X) be applied to calculate the leaf area of a tomato? Can you explain with an example and what is the substitution of Ln and ln?
06 August 2024 2,508 2 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...
05 August 2024 9,805 3 View
XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds
04 August 2024 3,019 3 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Machine learning (ML) has shown great potential in predicting the compressive strength of concrete, an important property for structural engineering. However, its practical application comes with...
03 August 2024 2,546 2 View
Hello to everyone, I'm trying to extrtact total RNA from sEVs. I made a first experiment starting from 2x10^10 sEVs quantified by NTA but the RNA obtained was very low (under 20ng/ml) Do you have...
18 June 2024 2,799 4 View
Hi, I am doing western blotting of isolated extracellular vesicles, and I have albumin and calnexin contamination that I don't know why. Is there any way to avoid it?
16 June 2024 9,907 4 View
Please give some insight on this
06 June 2024 2,016 2 View
Ultracentrifugation is the most widely used method for isolating extracellular vesicles (EVs), despite the significant structural damage that EVs suffer during the process.
16 May 2024 9,432 1 View
In the younger boy there is no ejaculate because the prostate and seminal vesicles are not yet functionally developed, and in the female those glands are rudimentary and never develop. (Alfred Kinsey)
29 April 2024 6,483 0 View
I tried to inhibit extracellular microvesicles(MVs) secreted from cells, through using Rock inhibitor(Y27632), calpeptin and several siRNAs(siCDC42, siTSG101, siARF1), but failed to decrease MVs....
23 April 2024 7,369 0 View
Dear Colleagues, We plan to isolate and characterise extracellular vesicles released by dying cells during radiotherapy of head and neck squamous cell carcinoma. Given the duration of...
14 April 2024 494 3 View
I am planning to study extracellular vesicles, and to do this I need cells with a fluorescent membrane (GFP, Tomato, etc.), do you guys have any suggestion of lentivirus vector? Thanks!
02 April 2024 1,944 0 View
Hello dearest EV people! I want to plate my isolated EVs on coated glass coverslips and image them with confocal microscopy for CDs and other EV markers. I wonder if anyone tried a normal IF...
21 March 2024 3,344 1 View
I found one publication detected CD9, CD63, and CD81 via western blot. I feel a little confused about it. The antibody they used are specific for human, mouse (I checked the product in the...
20 March 2024 9,277 1 View